A2780s cells were more susceptible to both cisplatin and carfilzomib while analyses of drug interactions between the two agents showed synergistic effects in all affected fractions of drugtreated a2780s and a2780cp cells ci0. Table s1 ic50 values for a0 and cisplatin in human cancer cells ic50. Anderson cancer center, 1515 holcombe boulevard, houston, tx 770304009, usa cisplatin is one of the most potent antitumor agents. Full text molecular mechanisms of cisplatin resistance in. The treatment of the cells with cisplatin or bromelain alone resulted in a dosedependent and a timedependent decrease in the viability of the mdamb231 cells at 24 and 48 h, with higher concentrations and longer treatment times resulting in lower levels of cell viability fig. The effects of cisplatin on transcription of e6 were analyzed by rtpcr. M, respectively p cisplatin in cervical cancer cell line hela and its mechanism, cell growth inhibition of cisplatin on hela cells was analyzed by mtt assay. Doseresponse curves for hela, siha, vero, and 3t3 cell openi. The aim of this study was to determine whether cisplatinresistant cells derived from hela cells have an apoptosisresistant phenotype.
Mechanisms of cisplatin resistance in triple negative breast. Hela cell proliferation the results from mtt assay showed a concentration and time dependent reduction in the proliferation rate of hela cells on treatment with cidanandcisplatin figure 1. Induction of cisplatin resistance in a2780 cell line cisplatin resistant a2780 cell lines were derived from original parental cell line by continuous exposure to stepwise increasing concentrations of cisplatin. We sought to investigate if gleevec has a potential role in treatment of nonsmall cell. Hypoxiainduced autophagy mediates cisplatin resistance in. Meanwhile, the ic 50 of thymoquinone in hela and siha cells was 29. Gleevec binds specifically to bcrabl tyrosine kinase and inhibit the tyrosine kinase activity. Jci acquired cisplatin resistance in human ovarian cancer. The genomics of drug sensitivity in cancer project is a collaboration between the cancer genome project at the wellcome sanger institute uk and the center for molecular therapeutics, massachusetts general hospital cancer center usa. Gleevec sti571 inhibits lung cancer cell growth a549 and. After transfection, the ic50 value of siha and hela cells was examined by cck 8. Preparation and cytotoxicity of cisplatincontaining liposomes. The total phenolics, cisplatin and docetaxel had significant inhibitory and apoptosispromoting effects on hela cells p cisplatin in vitro in hela cells cervical cancer cell line. For the lung is the firstline to contact with the atmosphere, in which the oxygen content is 21%, so 21% o2 is commonly used as.
Issues surrounding standard cytotoxicity testing for assesing. Cytotoxicity assays were performed on cells derived from fresh tumor explants of 18 human cases of malignant. The half maximal inhibitory concentration ic50 of cisplatin in hela and siha cells at 72 hours was. Cidan and cisplatin reduced the viability of hela cells at 48 h to 43. Tunable biodegradable nanocomposite hydrogel for improved cisplatin efficacy on hct116 colorectal cancer cells and decreased toxicity in. Apoptosis is the main response of cells to chemotherapeutic agents including cisplatin. A cisplatinresistant cell subline, hela cddp cells, showed a 19fold resistance to cisplatin compared with the parent cells. Potential implications of grp58 expression and susceptibility. Generation of cisplatinresistant ovarian cancer cell lines. Since the discovery of cisplatin more than 40 years ago and its clinical introduction in the 1970s an enormous amount of research has gone into elucidating the mechanism of action of cisplatin on tumor cells. To quantitate the sensitisation of cells to chemotherapeutics after infection with aav2, doseresponse curves were established.
The subline showed a collateral sensitivity to paclitaxel. Some experiments have found that cisplatin can inhibit protein expression in hela cells of cervical cancer cell lines, promote cell apoptosis significantly and inhibit the growth to achieve a. However, the resistant cell line was twofold more efficient at effluxing drug, which was associated with reduced total drug accumulation for equivalent. Then, cell viability was assessed using mtt assay and ic50 was determined. Systematic identification of genomic markers of drug. After the indicated incubation times in cisplatin media the cells were harvested with trypsinization and incubated in a propidium iodide cocktail 50 gml propidium iodide sigma, 5 gml rnase a, 0.
Every two months, cell survival was analyzed by mtt assay. Combination of cisplatin and cinnamon essential oil inhibits. Less than 30% of malignant gliomas respond to adjuvant chemotherapy. Ratio of cisplatin ic50 to compound ic50 for mouse b16f10. Synthesis and cytotoxicity evaluation of novel asymmetrical. Cinnamon essential oil, cisplatin, hela cells, apoptosis, cell cycle. Following the 48 and 72h hour treatments at 37c, cells were. The development of anticancer rutheniumii rsc publishing. The mechanism of cisplatininduced apoptosis in hela cells. Dipyridamole increases the cytotoxicity of cisplatin in human. Cisplatin has a number of side effects that can limit its use. Cisplatin activates sapkjnk, p38 kinase, and erk dose dependently in cisplatin sensitive hela cells, whereas cisplatin mediated activation of sapkjnk is significantly reduced in the cpr subline. Cisplatina resistant hela cells are resistant to apoptosis.
Cell death in hela cells upon imperatorin and cisplatin treatment. Relationship between ic 50 values and cell seeding densities. A cisplatin resistant cell subline, hela cddp cells, showed a 19fold resistance to cisplatin compared with the parent cells. With a novel cell biosensor chip system allowing continuous monitoring of respiration, glycolysis, and impedance we followed cisplatin treatment of different cancer cell lines in real. Percentage inhibition of a549 lung cancer cells by cisplatin. Increase in the concentration of cidan from 10 to 30 mg mlled to decrease in the proliferation rate of hela cells from 95. Jul 23, 2015 hypoxia enhanced the cisplatin resistance of lung cancer cells. M, respectively p cisplatin is particularly effective against testicular cancer. It crossreacts with another two important membrane tyrosine kinase receptors, ckit and pdgf receptors. Cytotoxicity cisplatinresistant tumor lines ic 50 m cancer cell line cisplatin 1 chronic myelogenous leukemia k562 80 3 chronic myelogenous leukemia kg1 48 2 acute myelogenous leukemia ml2 80 1 mouse melanoma b16 80 6 colon cancer ht29n 80 12 colon cancer ht29 50 8 colon cancer hct116 80 9 lung carcinoma a427 63 6 breast carcinoma. Platinum accumulation in both sensitive and resistant cell lines. Ci value of administration of metformin and cisplatin in hela cells. Covid19 is an emerging, rapidly evolving situation.
In addition, cisplatin induces apoptosis with the caspasedependent pathway. Cisplatin sensitivity is enhanced in nonsmall cell lung. The cytotoxicity was evaluated by sulforhodamine b assay against a549, b16f10, hct15, skmel2 and skov3 cell lines and compared with standard drug cisplatin. The changing 50% inhibitory concentration ic50 of cisplatin. Cisplatin and doxorubicin were added twice a week after reseeding. Thus, the inhibition of hela cells growth at 24 hours is likely through cell cycle modulation rather than apoptosis.
Jan 17, 2008 two models were developed from h69 sclc cells, h69. Enhanced sensitivity of small cell lung cancer cell lines to. Compound 5f outperformed cisplatin against a549, hct15, skmel2 and b16f10 cell lines. When studied at their respective ic50 doses, drug accumulation rates were similar for the two cell lines.
M, cisplatin sensitivity increased in both cell lines compared to cisplatin treatment alone. Combination of cisplatin and bromelain exerts synergistic. Cell apoptosis was examined by cytometry and hoechst33258 staining after treatment with cisplatin. Original article inhibition of the cervical cancer growth. Sensitivity to cisplatin in primary cell lines derived from. The cell growth inhibition of cisplatin on hela cells was analyzed by mtt assay. M cell line a 0 cisplatin shsy5y 50 2 pnt1 10 5 sw872 50 5 rd 21 6 a375 42 6 hep2 28 7 mcf7dx 50 7 ht1080 12 9 hos 26 10 saos2 50 11 pc3 34 12 hepg2 48 12 hela 16 mcf7 50 15 panc1 50 15 caco2 17 a431 22 20 u2os 34 20 ht29 50 22. Cisplatin doseresponse curve of hela cells treated in suspension with 4 pgml cisplatin for one to 4 h under aerobic a or hypoxic a conditions.
Cp and h69cis200 using 100 ngml or 200 ngml cisplatin respectively. This complex induced death in hela cells, with an ic50. We determined the relative proliferation of the cell lines after mockinfection pbs or aav2 infection with 10 3 or 10 4 tcidcell and subsequent treatment with various concentrations of cisplatin or etoposide or a combination of both drugs. Mar 28, 2012 cells from ewings sarcoma were more sensitive to olaparib p 2.
Inhibition of the cervical cancer growth by cidan and cisplatin. Meanwhile, the ic50 of thymoquinone in hela and siha cells was 29. The inhibitory effect of cisplatin on hela cell growth was analyzed by the mtt assay. The cytotoxicity of email protected coreshell nanocrystals, evaluated by mtt assay, shows a much lower ic50 35. An equitoxic dose ic50 of cisplatin produced dna fragmentation in hela cells but not in hela cddp cells. Cisplatin induces down regulation of bcl2 in t47d breast.
In cochlear cells, cisplatin treatment results in the increase of caspases3 and 9 and causes the cochlear damage side effect 1. Sphase arrest on hela cells, cisplatin induced g1 arrest, while combination of ceo and cisplatin induced g2m arrest. In the presence of 30 m dip, the ic 50 of cisplatin was reduced by 39% for both cell lines. There are some common chemotherapy drugs, such as cisplatin, docetaxel. Pdf metformin enhances antiproliferative effect of cisplatin in. Understanding cisplatin resistance using cellular models. Inhibitory concentration ic50 of cisplatin and metformin was determined before.
Cinnamon essential oil, cisplatin, hela cells, apoptosis, cell. Combination index ci was calculated to evaluate the. Cell viability was evaluated with the resazurin assay see materials and methods. Doseresponse curves for hela, siha, vero, and 3t3 cell. Selection of our books indexed in the book citation index in web of.
The effect of cisplatin on transcription of hpv e6 was analyzed by rtpcr and protein expression. The half maximal inhibitory concentration ic 50 of cisplatin in hela and siha cells at 72 hours was. The a2780cp70 cell line was fold more resistant to cisplatin than a2780 cells. Cisplatin was also found to cause ros generation and increase lipid peroxidation, which leads cells to the apoptotic pathway. Vogels textbook of practical organic chemistry, 5th edition. Gleevec aka sti571, imatinib is a recently fda approved antitumor drug for chronic myelogenous leukemia. In this study, t47d cells were treated with various concentrations of cisplatin 0, 3. The study of cytotoxicity to glc4 and glc4 cddp cell lines showed similar ic50 values approximately 1. Combination of ceo and cisplatin decreased cells viability rather than cisplatin solely fig. Cells were then treated with 100 l compound of concentrations between 6. P ic 50 of cisplatin for each cell line in rpmi 1640 containing 10% fbs and 1% penicillinstreptomycin solution. Mir1284 enhances sensitivity of cervical cancer cells to cisplatin. Combination of cisplatin and cinnamon essential oil.
Pdf the changing 50% inhibitory concentration ic50 of cisplatin. Here, we have asked whether variations in the constitutive expression of earlygrowth response factor 1 egr1 predicted acute cytotoxicity and clonogenic cell death in vitro, induced by six different chemotherapics. The concentrations able to inhibit 50% viability ic50 were calculated and cell lines were ordered according to the ic50 value for cisplatin. Ratio of cisplatin ic50 to compound ic50 for human a431 cells. When hela cells were irradiated in the presence of cisplatin under hypoxic conditions, the resulting c e d lo. Cell death in hela cells upon imperatorin and cisplatin treatment article pdf available in folia histochemica et cytobiologica 503.
Cell apoptosis was measured with flow cytometry and hoechst 33258 staining following cisplatin treatment. Combination assay was done by combined ceo and cisplatin on hela cells with serial concentrations of 120, 18, 14, and 12 ic 50 of ceo and cisplatin. Apexbio cisplatininhibits dna synthesis,chemotherapy drug. Cytotoxic activity of methanolic extract of artocarpus.
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